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Anti-CD39 [R22]

AB02890-23.0-BT
Absolute Antibody
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetENTPD1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD39 [R22]
  • Delivery Days Customer
    9
  • Application Supplier Note
    To study the expression and distribution of CD39, flow cytometry was performed on PBMCs and splenic e-cells using the mouse version of this antibody (Kansas et al, 1991; pmid:1672348).
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    R22
  • Gene ID953
  • Target name
    ENTPD1
  • Target description
    ectonucleoside triphosphate diphosphohydrolase 1
  • Target synonyms
    ATP-DPH, ATPDase, CD39, NTPDase-1, SPG64, ectonucleoside triphosphate diphosphohydrolase 1, ATP diphosphohydrolase, CD39 antigen, NTPDase1, ecto-ATP diphosphohydrolase 1, ecto-ATPDase 1, ecto-ATPase 1, ecto-apyrase, lymphoid cell activation antigen, nucleoside triphosphate diphosphohydrolase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49961
  • Protein Name
    Ectonucleoside triphosphate diphosphohydrolase 1
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format for improved compatibility with existing reagents assays and techniques.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD39/ENTPD1 using anti-CD39/ENTPD1 antibody (A03196-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD39/ENTPD1 antigen affinity purified polyclonal antibody (Catalog # A03196-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD39/ENTPD1 at approximately 80 kDa. The expected band size for CD39/ENTPD1 is at 80 kDa.
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