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Anti-CD4 [OX-35]

AB00567-23.0-BT
Absolute Antibody
ApplicationsFunctional Assay, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityRat
TargetCd4
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD4 [OX-35]
  • Delivery Days Customer
    9
  • Application Supplier Note
    This antibody is able to label cells expressing CD4 on their surface and can precipitate CD4 from detergent extracts of radioactively labelled thymocytes.
  • Applications
    Functional Assay, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OX-35
  • Gene ID24932
  • Target name
    Cd4
  • Target description
    Cd4 molecule
  • Target synonyms
    W3/25, p55, T-cell surface glycoprotein CD4, CD4 antigen, T-cell surface antigen T4/Leu-3, W3/25 antigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05540
  • Protein Name
    T-cell surface glycoprotein CD4
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD4 using anti-CD4 antibody (A00344-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates, Lane 2: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD4 antigen affinity purified polyclonal antibody (Catalog # A00344-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD4 at approximately 52KD. The expected band size for CD4 is at 52KD.
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