Anti-CD49B [GBR500 (Vatelizumab, TMC-2206)]

Absolute Antibody

Anti-CD49B [GBR500 (Vatelizumab, TMC-2206)]

9

The binding of this antibody to recombinant GST-human alpha2I domain fusion protein and intact alpha2beta1 integrin was evaluated using ELISA. It was reported that this antibody bound immobilized human alpha2beta1 integrin with a binding affinity in the nanomolar range. The reactivity of this antibody towards alpha2beta1 integrin expressed on blood cells from different species was evaluated by flow cytometry (US7807794). In vitro, this antibody inhibits the binding of PC3 prostate cancer cells to collagen with an EC50 of 0.035 microg/ml. To study the impact of VLA-2 on extravasation in a PC3-luc metastasis model, this antibody was used to block VLA-2 before injection of tumor cells and by pre-dosing mice with a 50 mg/kg dose i.v. Bi-weekly treatment with this antibody at 5 mg/kg successfully slowed tumor growth and was as effective as bevacizumab 5 mg/kg treatment (Attinger et al., Cancer Research 2011). A study involving orthotopic mouse xenograft model used a DU145-luc cell line and explored the importance of VLA-2 for tumor growth in a physiological setting. It was reported that VLA-2 inhibition leads to modest growth reduction in subcutaneous xenografts (Hou et al., Mol Cancer Ther 2011). In a phase 2 clinical study in multiple sclerosis patients, the safety and efficacy of this antibody was evaluated. Following treatment with this antibody enhanced frequencies of regulatory T cells (TREG) were reported (PMID: 30783682).

Flow Cytometry, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

GBR500 (Vatelizumab, TMC-2206)

ITGA2

integrin subunit alpha 2

BR, CD49B, FMAIT3, GPIa, HPA-5, VLA-2, VLAA2, integrin alpha-2, CD49 antigen-like family member B, alpha 2 integrin, alpha 2 subunit of VLA-2 receptor, collagen receptor, glycoprotein Ia, human platelet alloantigen 5, human platelet alloantigen system 5, integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor), platelet antigen Br, platelet glycoprotein GPIa, platelet membrane glycoprotein Ia, very late activation protein 2 receptor, alpha-2 subunit, very late activation receptor alpha-2 subunit, very late activation receptor subunit alpha-2

Mouse

IgG2a

Integrin alpha-2

This is a reformatted mouse IgG2a Fc Silent™ antibody, based on the original mouse IgG4 format, created for improved compatibility with existing reagents, assays and techniques.

Human, Monkey, Primate, Rat

-20°C,2°C to 8°C

12352203