Bio-Connect

Anti-CD62L [DREG-56]

Ab00622-1.1
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, Neutralisation/Blocking
Product group Antibodies
TargetSELL
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD62L [DREG-56]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody has been used in WB, IP and binding assays to define a lymphocyte homing receptor for PLN HEVs (Kishimoto, 1990). In vitro, this antibody has been shown to specifically inhibit over 90% of binding of human lymphocytes to HEVs within frozen sections of peripheral, but not mucosal, lymphoid tissue (Kishimoto, 1990). This antibody has also been used in labelling studies to demonstate that the trophectoderm of implantation-competent human embryos expresses L-selectin (Genbacev, 2003).
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, Neutralisation/Blocking
  • Applications Supplier
    IP; FC; WB; blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    DREG-56
  • Gene ID6402
  • Target name
    SELL
  • Target description
    selectin L
  • Target synonyms
    CD62 antigen-like family member L; CD62L; gp90-MEL; LAM1; LECAM1; LEU8; leukocyte surface antigen Leu-8; leukocyte-endothelial cell adhesion molecule 1; LNHR; LSEL; L-selectin; LYAM1; lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; PLNHR; TQ1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP14151
  • Protein Name
    L-selectin
  • Reactivity Supplier
    Human, Chimpanzee, Bovine
  • Reactivity Supplier Note
    This antibody was produced by immunizing mice with the concentrated supernatant generated after centrifugation of human peripheral blood leukocytes.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD62L using anti-CD62L antibody (A00652). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human CD62L protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD62L antigen affinity purified polyclonal antibody (Catalog # A00652) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD62L at approximately 90KD. The expected band size for CD62L is at 60KD.
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