
Figure 1. Western blot analysis of CD62L using anti-CD62L antibody (A00652). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human CD62L protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD62L antigen affinity purified polyclonal antibody (Catalog # A00652) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD62L at approximately 90KD. The expected band size for CD62L is at 60KD.
Anti-CD62L/SELL Antibody Picoband(r)
A00652-CARRIER-FREE
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSELL
Overview
- SupplierBoster Bio
- Product NameAnti-CD62L/SELL Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID6402
- Target nameSELL
- Target descriptionselectin L
- Target synonymsCD62 antigen-like family member L; CD62L; gp90-MEL; LAM1; LECAM1; LEU8; leukocyte surface antigen Leu-8; leukocyte-endothelial cell adhesion molecule 1; LNHR; LSEL; L-selectin; LYAM1; lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; PLNHR; TQ1
- HostRabbit
- IsotypeIgG
- Protein IDP14151
- Protein NameL-selectin
- Scientific DescriptionBoster Bio Anti-CD62L/SELL Antibody Picoband® catalog # A00652. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203