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ProductsBack/SELL Antibody
Western Blot analysis of K562 cells using L-Selectin Polyclonal Antibody
Western Blot analysis of K562 cells using L-Selectin Polyclonal Antibody
Western Blot analysis of K562 cells using L-Selectin Polyclonal Antibody

SELL Antibody

CSB-PA004858
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSELL
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Overview

  • Supplier
    Cusabio
  • Product Name
    SELL Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6402
  • Target name
    SELL
  • Target description
    selectin L
  • Target synonyms
    CD62L, LAM1, LECAM1, LEU8, LNHR, LSEL, LYAM1, PLNHR, TQ1, L-selectin, CD62 antigen-like family member L, gp90-MEL, leukocyte surface antigen Leu-8, leukocyte-endothelial cell adhesion molecule 1, lymph node homing receptor, lymphocyte adhesion molecule 1, pln homing receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14151
  • Protein Name
    L-selectin
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of CD62L using anti-CD62L antibody (A00652). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human CD62L protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD62L antigen affinity purified polyclonal antibody (Catalog # A00652) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD62L at approximately 90KD. The expected band size for CD62L is at 60KD.
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