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ProductsBack/Anti-CD63 [Nb17-17], Human IgG1-Fc Fusion,

Anti-CD63 [Nb17-17], Human IgG1-Fc Fusion,

AB04685-10.159
Absolute Antibody
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetCD63
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD63 [Nb17-17], Human IgG1-Fc Fusion,
  • Delivery Days Customer
    9
  • Application Supplier Note
    The original antibody was used for an ELISA on CD63-His to determine specificity (CN117736326A).
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    Nb17-17
  • Gene ID967
  • Target name
    CD63
  • Target description
    CD63 molecule
  • Target synonyms
    AD1, HOP-26, ME491, MLA1, OMA81H, Pltgp40, TSPAN30, CD63 antigen, AD1 antigen, CD63 antigen (melanoma 1 antigen), granulophysin, limp1, melanoma-associated antigen ME491, melanoma-associated antigen MLA1, ocular melanoma-associated antigen, tetraspanin-30, tspan-30
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP08962
  • Protein Name
    CD63 antigen
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Alpaca nanobody format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD63 using anti-CD63 antibody (PB9250). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MDA-MB-453 whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD63 antigen affinity purified polyclonal antibody (Catalog # PB9250) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD63 at approximately 30-60 kDa. The expected band size for CD63 is at 26 kDa.
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