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Figure 1. Western blot analysis of CD68 using anti-CD68 antibody (PA1518). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD68 antigen affinity purified polyclonal antibody (Catalog # PA1518) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD68 at approximately 90-100 kDa. The expected band size for CD68 is at 37 kDa.
Figure 1. Western blot analysis of CD68 using anti-CD68 antibody (PA1518). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD68 antigen affinity purified polyclonal antibody (Catalog # PA1518) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD68 at approximately 90-100 kDa. The expected band size for CD68 is at 37 kDa.
Figure 1. Western blot analysis of CD68 using anti-CD68 antibody (PA1518). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD68 antigen affinity purified polyclonal antibody (Catalog # PA1518) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD68 at approximately 90-100 kDa. The expected band size for CD68 is at 37 kDa.

Anti-Macrosialin CD68 Antibody Picoband(r)

PA1518
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetCd68
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-CD68 Antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHP, IHF, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Gene ID12514
  • Target name
    Cd68
  • Target description
    CD68 antigen
  • Target synonyms
    gp11; gp110; La; Lamp4; ma; macrosialin; Sca; Scard1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31996
  • Protein Name
    Macrosialin
  • Scientific Description
    Superstar antibody: Bosters anti mouse CD68 antibody (PA1518) is among its top 10 bestselling antibodies, and one of the most cited mouse/rat CD68 antibodies on the market. Because of this antibodys high demand, we have subsequently developed a rabbit monoclonal anti CD68 antibody (M00602-1) targetting a similar epitope. High specificity: PA1518 reacts with murine macrosialin/CD68, and has been validated with Western blotting and confirmed its stellar specificity. CD68 is a highly glycosylated protein, and its expected western blot molecular weight is between 80kDa to 110kDa, depending on glycosylation level. PA1518s observed MW in WB is near 90-100kDa. Testing on negative control tissues showed no significant bands (images available on request) or staining. See more info in the positive and negative control design section. Based on immunogen sequence homology, this antibody is not expected to cross react with other proteins from the LAMP family, which complies with QC testing observations. Great for CD68 IHC: This antibody produces clean and specific immunobiological stains in both mouse and rat tissues. Our QC team has validated it on spleen and liver tissues of both mice and rats. Click product images for more details on experiment conditions. More about CD68: CD68 is a cell surface marker often used to identify macrophages and other cell types in the monocyte lineage. It is a transmembrane protein that binds to electins and selectins and plays a role in macrophage homing movement. Check out the CD68 biomarker page for more information on CD68 and view all CD68 antibodies, ELISA kits and proteins. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity Supplier
    Mouse, Rat, Hamster
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD68 using anti-CD68 antibody (PA1518). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD68 antigen affinity purified polyclonal antibody (Catalog # PA1518) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD68 at approximately 90-100 kDa. The expected band size for CD68 is at 37 kDa.
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