More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/anti-CD8 (human), mAb (14) (APC)
Binding of anti-CD8/APC to human PBL. Methods: Five x 10^5 ficoll-prepared human peripheral blood leucocytes were washed and pre-incubated with 20microl of 300microg/ml human IgG (to reduce non-specific binding) after which they were incubated 45 min on ice with 80microl of anti-CD8/APC at a 1:50 dilution (10microg/ml). Cells were washed three times, fixed and analyzed by FACS using a lymphoid gate. A 10.3% subpopulation of the cells stained positive with a mean shift of 2.1 log10 fluorescent units when compared to Isotype Control Mouse IgG1/APC (#ANC-278-060). Binding was blocked when cells were pre-incubated 10 min with 20microl of 0.5mg/ml anti-CD8(14) antibody (#ANC-154-020).
Binding of anti-CD8/APC to human PBL. Methods: Five x 10^5 ficoll-prepared human peripheral blood leucocytes were washed and pre-incubated with 20microl of 300microg/ml human IgG (to reduce non-specific binding) after which they were incubated 45 min on ice with 80microl of anti-CD8/APC at a 1:50 dilution (10microg/ml). Cells were washed three times, fixed and analyzed by FACS using a lymphoid gate. A 10.3% subpopulation of the cells stained positive with a mean shift of 2.1 log10 fluorescent units when compared to Isotype Control Mouse IgG1/APC (#ANC-278-060). Binding was blocked when cells were pre-incubated 10 min with 20microl of 0.5mg/ml anti-CD8(14) antibody (#ANC-154-020).
Binding of anti-CD8/APC to human PBL. Methods: Five x 10^5 ficoll-prepared human peripheral blood leucocytes were washed and pre-incubated with 20microl of 300microg/ml human IgG (to reduce non-specific binding) after which they were incubated 45 min on ice with 80microl of anti-CD8/APC at a 1:50 dilution (10microg/ml). Cells were washed three times, fixed and analyzed by FACS using a lymphoid gate. A 10.3% subpopulation of the cells stained positive with a mean shift of 2.1 log10 fluorescent units when compared to Isotype Control Mouse IgG1/APC (#ANC-278-060). Binding was blocked when cells were pre-incubated 10 min with 20microl of 0.5mg/ml anti-CD8(14) antibody (#ANC-154-020).

anti-CD8 (human), mAb (14) (APC)

ANC-154-060
Ancell Corporation
ApplicationsFlow Cytometry, ELISA
Product group Antibodies
ReactivityHuman
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Ancell Corporation
  • Product Name
    anti-CD8 (human), mAb (14) (APC)
  • Delivery Days Customer
    10
  • Antibody Specificity
    Clone 14 recognizes the CD8 alpha chain of approximately 33 kda.
  • Applications
    Flow Cytometry, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    14
  • Concentration
    0.5 mg/ml
  • Conjugate
    APC (Allophycocyanin)
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Human CD8 is a heterodimeric protein consisting of an alpha and beta chain, each about 33kDa. CD8 is expressed on most thymocytes and on about one third of peripheral blood T cells. CD8 is a co-receptor involved in antigen recognition and binds to the alpha2 and alpha3 domain of MHC Class I molecules. - Monoclonal Antibody. Clone 14 recognizes the CD8 alpha chain of approximately 33 kda. Isotype: Mouse IgG1kappa. Immunogen: Human thymocytes. Applications: ELISA, FACS. Human CD8 is a heterodimeric protein consisting of an alpha and beta chain, each about 33kDa. CD8 is expressed on most thymocytes and on about one third of peripheral blood T cells. CD8 is a co-receptor involved in antigen recognition and binds to the alpha2 and alpha3 domain of MHC Class I molecules.
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Ancell Corporation
anti-CD8 (human), mAb (14)ANC-154-020
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
  • SizePrice
Product group Antibodies
Ancell Corporation
anti-CD8 (human), mAb (14) (Biotin)ANC-154-030
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
  • SizePrice
Product group Antibodies
Ancell Corporation
anti-CD8 (human), mAb (14) (FITC)ANC-154-040
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
  • SizePrice
Product group Antibodies
Ancell Corporation
anti-CD8 (human), mAb (14) (R-PE)ANC-154-050
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
  • SizePrice
Product group Antibodies
Ancell Corporation
anti-CD8 (human), mAb (14) (PE-Cy7)ANC-154-070
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
  • SizePrice
Product group Antibodies
Ancell Corporation
anti-CD8 (human), mAb (14) (preservative free)ANC-154-820
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
  • SizePrice