ProductsBack/anti-CD8 (human), mAb (14) (APC)

Binding of anti-CD8/APC to human PBL. Methods: Five x 10^5 ficoll-prepared human peripheral blood leucocytes were washed and pre-incubated with 20microl of 300microg/ml human IgG (to reduce non-specific binding) after which they were incubated 45 min on ice with 80microl of anti-CD8/APC at a 1:50 dilution (10microg/ml). Cells were washed three times, fixed and analyzed by FACS using a lymphoid gate. A 10.3% subpopulation of the cells stained positive with a mean shift of 2.1 log10 fluorescent units when compared to Isotype Control Mouse IgG1/APC (#ANC-278-060). Binding was blocked when cells were pre-incubated 10 min with 20microl of 0.5mg/ml anti-CD8(14) antibody (#ANC-154-020).

anti-CD8 (human), mAb (14) (APC)

ANC-154-060

Product group Antibodies

Overview

Supplier
Ancell Corporation
Product Name
anti-CD8 (human), mAb (14) (APC)
Delivery Days Customer
10
Certification
Research Use Only
Clonality
Monoclonal
Scientific Description
Human CD8 is a heterodimeric protein consisting of an alpha and beta chain, each about 33kDa. CD8 is expressed on most thymocytes and on about one third of peripheral blood T cells. CD8 is a co-receptor involved in antigen recognition and binds to the alpha2 and alpha3 domain of MHC Class I molecules. - Monoclonal Antibody. Clone 14 recognizes the CD8 alpha chain of approximately 33 kda. Isotype: Mouse IgG1kappa. Immunogen: Human thymocytes. Applications: ELISA, FACS. Human CD8 is a heterodimeric protein consisting of an alpha and beta chain, each about 33kDa. CD8 is expressed on most thymocytes and on about one third of peripheral blood T cells. CD8 is a co-receptor involved in antigen recognition and binds to the alpha2 and alpha3 domain of MHC Class I molecules.
Storage Instruction
2°C to 8°C
UNSPSC
12352203

anti-CD8 (human), mAb (14) (APC)

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