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ProductsBack/Anti-CD8a [C6], Rabbit IgG, kappa

Anti-CD8a [C6], Rabbit IgG, kappa

AB04596-23.0-BT
Absolute Antibody
ApplicationsELISA
Product group Antibodies
ReactivityRabbit
TargetCd8a
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD8a [C6], Rabbit IgG, kappa
  • Delivery Days Customer
    9
  • Application Supplier Note
    The original antibody was used for an ELISA on rabbit CD8a. This antibody was used for flow cytometry on rabbit cells. The antibody was used for a western blot on rabbit CD8a (CN116675772A).
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    C6
  • Gene ID12525
  • Target name
    Cd8a
  • Target description
    CD8 subunit alpha
  • Target synonyms
    Ly-2, Ly-35, Ly-B, Lyt-2, T-cell surface glycoprotein CD8 alpha chain, CD8 antigen, alpha chain, Lyt-2.1 lymphocyte differentiation antigen (AA at 100), T-cell surface glycoprotein Lyt-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01731
  • Protein Name
    T-cell surface glycoprotein CD8 alpha chain
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD8 alpha using anti-CD8 alpha antibody (A02236-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysate, Lane 2: rat thymus tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD8 alpha antigen affinity purified polyclonal antibody (Catalog # A02236-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD8 alpha at approximately 36KD. The expected band size for CD8 alpha is at 26KD.
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