ProductsBack/Anti-CD9 Antibody

Anti-CD9 Antibody

144-01703

ApplicationsWestern Blot

Product group Antibodies

TargetCD9

Overview

Supplier
RayBiotech
Product Name
Anti-CD9 Antibody
Delivery Days Customer
16
Applications
Western Blot
Certification
Research Use Only
Clonality
Polyclonal
Conjugate
Unconjugated
Gene ID928
Target name
CD9
Target description
CD9 molecule
Target synonyms
5H9 antigen; antigen CD9; BA-2/p24 antigen; BTCC-1; CD9 antigen; CD9 antigen (p24); cell growth-inhibiting gene 2 protein; DRAP-27; leukocyte antigen MIC3; MIC3; motility related protein-1; MRP-1; tetraspanin-29; TSPAN29; TSPAN-29
Host
Rabbit
Isotype
IgG
Protein IDP21926
Protein Name
CD9 antigen
Scientific Description
CD9 Polyclonal Antibody
Storage Instruction
-20°C
UNSPSC
12352203

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Figure 1. Western blot analysis of CD9 using anti-CD9 antibody (A01202-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD9 antigen affinity purified polyclonal antibody (Catalog # A01202-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD9 at approximately 22 kDa. The expected band size for CD9 is at 25 kDa.

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$RSC96 cells probed with\tCD9/MRP-1 Polyclonal Antibody, Unconjugated (bs-2489R ) at 0.2ug for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).$

Product group Antibodies

References

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Anti-CD9 Antibody

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Overview

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