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ProductsBack/CD9 Antibody (clone HI9a)

CD9 Antibody (clone HI9a)

LS-C772815
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence
Product group Antibodies
TargetCD9
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD9 Antibody (clone HI9a)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is less than or equal to 0.5 microg per 10E6 cells in 100 microl volume or 100 microl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.. Flo, IF Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is less than or equal to 0.5 µg per 10E6 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
  • Applications
    Flow Cytometry, ImmunoFluorescence
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HI9a
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID928
  • Target name
    CD9
  • Target description
    CD9 molecule
  • Target synonyms
    5H9 antigen; antigen CD9; BA-2/p24 antigen; BTCC-1; CD9 antigen; CD9 antigen (p24); cell growth-inhibiting gene 2 protein; DRAP-27; leukocyte antigen MIC3; MIC3; motility related protein-1; MRP-1; tetraspanin-29; TSPAN29; TSPAN-29
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD9 using anti-CD9 antibody (A01202-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD9 antigen affinity purified polyclonal antibody (Catalog # A01202-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD9 at approximately 22 kDa. The expected band size for CD9 is at 25 kDa.
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