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Immunohistochemical staining of human pancreas shows strong membranous positivity in islets of Langerhans.
Immunohistochemical staining of human pancreas shows strong membranous positivity in islets of Langerhans.
Immunohistochemical staining of human pancreas shows strong membranous positivity in islets of Langerhans.

Anti-CD99-25ul

HPA035304
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CD99-25ul
  • Delivery Days Customer
    6
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14209
  • Protein Name
    CD99 antigen
  • Scientific Description
    Rabbit Polyclonal Anti-CD99 Antibody against Human CD99 molecule. Validated for Immunohistochemistry and Western Blot
  • Reactivity
    Human
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD99 using anti-CD99 antibody (A01724-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD99 antigen affinity purified polyclonal antibody (Catalog # A01724-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD99 at approximately 28 kDa. The expected band size for CD99 is at 28 kDa.
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