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Immunohistochemical staining of human kidney shows strong membranous positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong membranous positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong membranous positivity in cells in tubules.

Anti-CDH6 Antibody

HPA007456
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetCDH6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CDH6
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1004
  • Target name
    CDH6
  • Target description
    cadherin 6
  • Target synonyms
    CAD6; cadherin 6, type 2, K-cadherin (fetal kidney); cadherin-6; KCAD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55285
  • Protein Name
    Cadherin-6
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of K Cadherin/CDH6 using anti-K Cadherin/CDH6 antibody (A06353-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-K Cadherin/CDH6 antigen affinity purified polyclonal antibody (Catalog # A06353-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for K Cadherin/CDH6 at approximately 88KD. The expected band size for K Cadherin/CDH6 is at 88KD.
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