ProductsBack/Mouse anti K-Cadherin/Cadherin-6

Mouse anti K-Cadherin/Cadherin-6

MUB0305P

ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Product group Antibodies

TargetCDH6

Overview

Supplier
Nordic-MUbio
Product Name
Mouse anti K-Cadherin/Cadherin-6
Delivery Days Customer
7
Application Supplier Note
2B6 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen and paraffin sections when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. Optimal antibody dilution should be determined by titration; recommended range is 1:25 - 1:50 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:25 - 1:250 for immunoblotting applications.
Applications
Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Applications Supplier
Immunocytochemistry;Immunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting
Category Supplier
Primary antibodies
Certification
Research Use Only
Clonality
Monoclonal
Clone ID
2B6
Gene ID1004
Target name
CDH6
Target description
cadherin 6
Target synonyms
CAD6; cadherin 6, type 2, K-cadherin (fetal kidney); cadherin-6; KCAD
Host
Mouse
Isotype
IgG1
Protein IDP55285
Protein Name
Cadherin-6
Source
2B6 is a Mouse monoclonal IgG1 antibody obtained by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with affinity purified extracellular domain of Human cadherin-6-GST fusion protein.
Reactivity Supplier
Human;Rat
UNSPSC
12352203

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Figure 1. Western blot analysis of K Cadherin/CDH6 using anti-K Cadherin/CDH6 antibody (A06353-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-K Cadherin/CDH6 antigen affinity purified polyclonal antibody (Catalog # A06353-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for K Cadherin/CDH6 at approximately 88KD. The expected band size for K Cadherin/CDH6 is at 88KD.

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$Images provided by the Independent Validation Program badge number 29743.\n\nHistogram of MCF-7 cells stained with Rabbit Anti-K-Cadherin Polyclonal Antibody (bs-5823R at 1:100)(orange),isotype control antibody (green),secondary antibody only (blue) and unstained (red).$