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Western Blot analysis of BT474 A549 SHSY5Y 293 cells using K-cadherin Polyclonal Antibody
Western Blot analysis of BT474 A549 SHSY5Y 293 cells using K-cadherin Polyclonal Antibody
Western Blot analysis of BT474 A549 SHSY5Y 293 cells using K-cadherin Polyclonal Antibody

CDH6 Antibody

CSB-PA004767
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCDH6
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Overview

  • Supplier
    Cusabio
  • Product Name
    CDH6 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1004
  • Target name
    CDH6
  • Target description
    cadherin 6
  • Target synonyms
    CAD6, KCAD, cadherin-6, cadherin 6, type 2, K-cadherin (fetal kidney)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55285
  • Protein Name
    Cadherin-6
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of K Cadherin/CDH6 using anti-K Cadherin/CDH6 antibody (A06353-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-K Cadherin/CDH6 antigen affinity purified polyclonal antibody (Catalog # A06353-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for K Cadherin/CDH6 at approximately 88KD. The expected band size for K Cadherin/CDH6 is at 88KD.
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Images provided by the Independent Validation Program badge number 29743.\n\nHistogram of MCF-7 cells stained with Rabbit Anti-K-Cadherin Polyclonal Antibody (bs-5823R at 1:100)(orange),isotype control antibody (green),secondary antibody only (blue) and unstained (red).
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