ProductsBack/CDH6 Antibody

Western Blot analysis of BT474 A549 SHSY5Y 293 cells using K-cadherin Polyclonal Antibody

CDH6 Antibody

CSB-PA004767

ApplicationsWestern Blot, ELISA

Product group Antibodies

TargetCDH6

Overview

Supplier
Cusabio
Product Name
CDH6 Antibody
Delivery Days Customer
20
Applications
Western Blot, ELISA
Certification
Research Use Only
Clonality
Polyclonal
Conjugate
Unconjugated
Gene ID1004
Target name
CDH6
Target description
cadherin 6
Target synonyms
CAD6; cadherin 6, type 2, K-cadherin (fetal kidney); cadherin-6; KCAD
Host
Rabbit
Isotype
IgG
Protein IDP55285
Protein Name
Cadherin-6
Storage Instruction
-20°C or -80°C
UNSPSC
12352203

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Figure 1. Western blot analysis of K Cadherin/CDH6 using anti-K Cadherin/CDH6 antibody (A06353-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-K Cadherin/CDH6 antigen affinity purified polyclonal antibody (Catalog # A06353-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for K Cadherin/CDH6 at approximately 88KD. The expected band size for K Cadherin/CDH6 is at 88KD.

Product group Antibodies

Anti-K Cadherin/CDH6 Antibody Picoband(r)A06353-1-CARRIER-FREE

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$Images provided by the Independent Validation Program badge number 29743.\n\nHistogram of MCF-7 cells stained with Rabbit Anti-K-Cadherin Polyclonal Antibody (bs-5823R at 1:100)(orange),isotype control antibody (green),secondary antibody only (blue) and unstained (red).$