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Anti-CEACAM1 [B3-17]

Ab01369-10.3
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCEACAM1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CEACAM1 [B3-17]
  • Delivery Days Customer
    7
  • Application Supplier Note
    In flow cytometric analysis, this antibody has been used to detect CEACAM1 surface expression by human epithelial and endothelial cell lines, and microvesicles derived from these cells (Muturi et al, 2013), normal human bronchial epithelial (NHBE) cells (Klaile et al, 2013), CEACAM1-knockout human intestinal epithelial cells (Klaile et al, 2017) and human B-cell subpopulations (Khairnar et al, 2015; Seifert et al, 2015). It has also been used to detect CEACAM1 expression in ELISA analysis of transfected A549 epithelial cells (Singer et al, 2014). This antibody has been used in immunofluorescence analysis of NHBE cells (Klaile et al, 2013), as well as in the immunoprecipitation of CEACAM1 from NHBE cell culture supernatants (Klaile et al, 2013). Additionally, this antibody has been used in IHC staining of paraffin-embedded human lung tissue sections (Klaile et al, 2013).
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    IP; IHC; IF; FC; ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    B3-17
  • Gene ID634
  • Target name
    CEACAM1
  • Target description
    CEA cell adhesion molecule 1
  • Target synonyms
    antigen CD66; BGP; BGP1; BGPI; carcinoembryonic antigen related cell adhesion molecule 1; carcinoembryonic antigen-related cell adhesion molecule 1; carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein); CD66a antigen
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP13688
  • Protein Name
    Carcinoembryonic antigen-related cell adhesion molecule 1
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This antibody was raised by immunising mice with recombinant human CEACAM1.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CEACAM1 using anti-CEACAM1 antibody (A00923-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG22 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM1 antigen affinity purified polyclonal antibody (Catalog # A00923-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEACAM1 at approximately 120-150 kDa. The expected band size for CEACAM1 is at 58 kDa.
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