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ProductsBack/Anti-CEACAM1 [C5-1X/8]

Anti-CEACAM1 [C5-1X/8]

AB01368-1.1-BT
Absolute Antibody
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCEACAM1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CEACAM1 [C5-1X/8]
  • Delivery Days Customer
    9
  • Application Supplier Note
    This antibody has been used in ELISA analysis to detect and quantify soluble CEACAM1 in human bronchoalveolar lavage fluid samples (Klaile et al, 2013). This antibody has also been used in immunohistochemical staining of paraffin-embedded human brain tissue sections, revealing CEACAM1-positive B cells in brain infiltrates (Rovituso et al, 2016). Additionally, this antibody has been used in Western blot analysis of CEACAM1 expression in human intestinal epithelial cell lysates (Klaile et al, 2017). This antibody does not inhibit B cell aggregation in vitro (Rovituso et al, 2016).
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    C5-1X/8
  • Gene ID634
  • Target name
    CEACAM1
  • Target description
    CEA cell adhesion molecule 1
  • Target synonyms
    BGP, BGP1, BGPI, cell adhesion molecule CEACAM1, CD66a antigen, antigen CD66, carcinoembryonic antigen related cell adhesion molecule 1, carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP13688
  • Protein Name
    Carcinoembryonic antigen-related cell adhesion molecule 1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CEACAM1 using anti-CEACAM1 antibody (A00923-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG22 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM1 antigen affinity purified polyclonal antibody (Catalog # A00923-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEACAM1 at approximately 120-150 kDa. The expected band size for CEACAM1 is at 58 kDa.
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