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ProductsBack/Anti-CEACAM1 / CD66a Reference Antibody (Chaim Sheba Med. Cntr. patent anti-CEACAM1)

Anti-CEACAM1 / CD66a Reference Antibody (Chaim Sheba Med. Cntr. patent anti-CEACAM1)

ORB1805853
Biorbyt
ApplicationsFunctional Assay, Flow Cytometry, ELISA, Other Application
Product group Antibodies
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Anti-CEACAM1 / CD66a Reference Antibody (Chaim Sheba Med. Cntr. patent anti-CEACAM1)
  • Delivery Days Customer
    10
  • Applications
    Functional Assay, Flow Cytometry, ELISA, Other Application
  • Applications Supplier
    ELISA, FA, FACS, Kinetics
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    >95%
  • Isotype
    IgG
  • Scientific Description
    Anti-CEACAM1 / CD66a Reference Antibody is expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CEACAM1 using anti-CEACAM1 antibody (A00923-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG22 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM1 antigen affinity purified polyclonal antibody (Catalog # A00923-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEACAM1 at approximately 120-150 kDa. The expected band size for CEACAM1 is at 58 kDa.
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