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Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.

Anti-CEBPD Antibody

HPA067581
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetCEBPD
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CEBPD
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1052
  • Target name
    CEBPD
  • Target description
    CCAAT enhancer binding protein delta
  • Target synonyms
    C/EBP-delta, CELF, CRP3, NF-IL6-beta, CCAAT/enhancer-binding protein delta, CCAAT/enhancer binding protein (C/EBP), delta, c/EBP delta, nuclear factor NF-IL6-beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49716
  • Protein Name
    CCAAT/enhancer-binding protein delta
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of CEBP Delta/CEBPD using anti-CEBP Delta/CEBPD antibody (A03499-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human U87 whole cell lysates, Lane 4: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEBP Delta/CEBPD antigen affinity purified polyclonal antibody (Catalog # A03499-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEBP Delta/CEBPD at approximately 36KD. The expected band size for CEBP Delta/CEBPD is at 36KD.
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