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ProductsBack/C/EBP delta antibody [N1C3]

C/EBP delta antibody [N1C3]

GTX115047
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
Product group Antibodies
TargetCEBPD
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Overview

  • Supplier
    GeneTex
  • Product Name
    C/EBP delta antibody [N1C3]
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.58 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1052
  • Target name
    CEBPD
  • Target description
    CCAAT enhancer binding protein delta
  • Target synonyms
    C/EBP-delta, CELF, CRP3, NF-IL6-beta, CCAAT/enhancer-binding protein delta, CCAAT/enhancer binding protein (C/EBP), delta, c/EBP delta, nuclear factor NF-IL6-beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49716
  • Protein Name
    CCAAT/enhancer-binding protein delta
  • Scientific Description
    The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-alpha. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses, and may be involved in the regulation of genes associated with activation and/or differentiation of macrophages. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Pentraxin 3 Facilitates Shrimp-Allergic Responses in IgE-Activated Mast Cells.
    Read more
  • C/EBPdelta drives key endocrine signals in the human amnion at parturition. Lu JW et al., 2021 Jun, Clin Transl Med
    Read more
  • Clinacanthus nutans Mitigates Neuronal Apoptosis and Ischemic Brain Damage Through Augmenting the C/EBPbeta-Driven PPAR-gamma Transcription. Wu JS et al., 2018 Jul, Mol Neurobiol
    Read more
  • CCAAT/enhancer-binding protein delta mediates tumor necrosis factor alpha-induced Aurora kinase C transcription and promotes genomic instability. Wu SR et al., 2011 Aug 19, J Biol Chem
    Read more

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Figure 1. Western blot analysis of CEBP Delta/CEBPD using anti-CEBP Delta/CEBPD antibody (A03499-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human U87 whole cell lysates, Lane 4: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEBP Delta/CEBPD antigen affinity purified polyclonal antibody (Catalog # A03499-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEBP Delta/CEBPD at approximately 36KD. The expected band size for CEBP Delta/CEBPD is at 36KD.
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