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Immunohistochemical staining of human small intestine shows strong plasma positivity.
Immunohistochemical staining of human small intestine shows strong plasma positivity.
Immunohistochemical staining of human small intestine shows strong plasma positivity.

Anti-CFH Antibody

HPA049176
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetCFH
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CFH
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3075
  • Target name
    CFH
  • Target description
    complement factor H
  • Target synonyms
    adrenomedullin binding protein; age-related maculopathy susceptibility 1; AHUS1; AMBP1; ARMD4; ARMS1; beta-1H; beta-1-H-globulin; CFHL3; complement factor H; factor H; factor H-like 1; FH; FHL1; H factor 1 (complement); H factor 2 (complement); HF; HF1; HF2; HUS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08603
  • Protein Name
    Complement factor H
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Factor H/CFH using anti-Factor H/CFH antibody (A00562-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCP tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor H/CFH antigen affinity purified polyclonal antibody (Catalog # A00562-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Factor H/CFH at approximately 150 kDa. The expected band size for Factor H/CFH is at 139 kDa.
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