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Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Factor H Polyclonal Antibody, Unconjugated (bs-9525R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Factor H Polyclonal Antibody, Unconjugated (bs-9525R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Factor H Polyclonal Antibody, Unconjugated (bs-9525R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.

CFH Polyclonal Antibody

BS-9525R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCFH
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Overview

  • Supplier
    Bioss
  • Product Name
    CFH Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF()
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3075
  • Target name
    CFH
  • Target description
    complement factor H
  • Target synonyms
    AHUS1, AMBP1, ARMD4, ARMS1, CFHL3, FH, FHL1, HF, HF1, HF2, HUS, complement factor H, H factor 1 (complement), H factor 2 (complement), adrenomedullin binding protein, age-related maculopathy susceptibility 1, beta-1-H-globulin, beta-1H, factor H, factor H-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08603
  • Protein Name
    Complement factor H
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Chen C, Lv Y, Hu C, et al. Alternative complement pathway is activated in the brains of scrapie-infected rodents. Med Microbiol Immunol. 2020,209(1):81-94. doi: 10.1007/s00430-019-00641-6
    Read this paper
  • Zhang Z, Fan X, Xi H, et al. Effect of local scrotal heating on the expression of tight junction-associated molecule Occludin in boar testes. Reprod Domest Anim. 2018,53(2):458-462. doi: 10.1111/rda.13131
    Read this paper
  • Wang XL, Hou L, Zhao CG, et al. Screening of genes involved in epithelial-mesenchymal transition and differential expression of complement-related genes induced by PAX2 in renal tubules. Nephrology (Carlton). 2019,24(2):263-271. doi: 10.1111/nep.13216
    Read this paper

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Figure 1. Western blot analysis of Factor H/CFH using anti-Factor H/CFH antibody (A00562-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCP tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor H/CFH antigen affinity purified polyclonal antibody (Catalog # A00562-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Factor H/CFH at approximately 150 kDa. The expected band size for Factor H/CFH is at 139 kDa.
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