More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/CFH Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Factor H Polyclonal Antibody, Unconjugated (bs-9525R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Factor H Polyclonal Antibody, Unconjugated (bs-9525R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Factor H Polyclonal Antibody, Unconjugated (bs-9525R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.

CFH Polyclonal Antibody

BS-9525R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCFH
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    CFH Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(ELISA=1:5000-10000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:100-500), IF(IF=1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3075
  • Target name
    CFH
  • Target description
    complement factor H
  • Target synonyms
    AHUS1, AMBP1, ARMD4, ARMS1, CFHL3, FH, FHL1, HF, HF1, HF2, HUS, complement factor H, H factor 1 (complement), H factor 2 (complement), adrenomedullin binding protein, age-related maculopathy susceptibility 1, beta-1-H-globulin, beta-1H, factor H, factor H-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08603
  • Protein Name
    Complement factor H
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

References

  • Alternative complement pathway is activated in the brains of scrapie-infected rodents. Chen C et al., 2020 Feb, Med Microbiol Immunol
    Read this paper
  • Effect of local scrotal heating on the expression of tight junction-associated molecule Occludin in boar testes. Zhang Z et al., 2018 Apr, Reprod Domest Anim
    Read this paper
  • Screening of genes involved in epithelial-mesenchymal transition and differential expression of complement-related genes induced by PAX2 in renal tubules. Wang XL et al., 2019 Feb, Nephrology (Carlton)
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Absolute Antibody
Anti-Factor H [OX23]AB00117-1.1-BT
ApplicationsImmunoPrecipitation, Western Blot, ELISA, Neutralisation/Blocking
ReactivityHuman, Mouse, Primate
TargetCFH
  • SizePrice
Product group Antibodies
Biosynth
Factor H antibody20C-CR6027SP
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CFH Antibody144-65490
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetCFH
  • SizePrice
Anti-CFH Antibody
Product group Antibodies
Antibodies.com
Anti-CFH AntibodyA30162
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CFH / Complement Factor H Antibody (clone 02, Preservative Free)LS-C819837
ApplicationsELISA
ReactivityHuman
TargetCFH
  • SizePrice
Figure 1. Western blot analysis of Factor H/CFH using anti-Factor H/CFH antibody (A00562-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCP tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor H/CFH antigen affinity purified polyclonal antibody (Catalog # A00562-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Factor H/CFH at approximately 150 kDa. The expected band size for Factor H/CFH is at 139 kDa.
Product group Antibodies
Boster Bio
Anti-Factor H/CFH Antibody Picoband(r)A00562-3-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetCFH
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-Complement factor HEB06848
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCFH
  • SizePrice
Product group Antibodies
Biomatik
CFH Polyclonal AntibodyCAC13012
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetCFH
  • SizePrice
Western blot All lanes: CFH antibody at 3microg/ml + human serum Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 140, 52 kDa Observed band size: 140 kDa
Product group Antibodies
Cusabio
CFH AntibodyCSB-PA005273ESR2HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCFH
  • SizePrice
Immunohistochemical analysis of paraffin-embedded U373 xenograft, using Factor H(GTX100305) antibody at 1:200 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Product group Antibodies
GeneTex
Factor H antibody [N3C1], InternalGTX100305
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetCFH
  • SizePrice