More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-Factor H [OX23]

Anti-Factor H [OX23]

AB00117-1.1-BT
Absolute Antibody
ApplicationsImmunoPrecipitation, Western Blot, ELISA, Neutralisation/Blocking
Product group Antibodies
TargetCFH
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Factor H [OX23]
  • Delivery Days Customer
    9
  • Application Supplier Note
    This antibody binds to Factor H and has been used to determine Factor H levels in a sandwich ELISA assay (Daniels-Treffandier, 2016). Alone it has no effect on Factor H activity, but it is able to reduce the increase in cofactor activity mediated by the antibody clone OX24.
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OX23
  • Gene ID3075
  • Target name
    CFH
  • Target description
    complement factor H
  • Target synonyms
    adrenomedullin binding protein; age-related maculopathy susceptibility 1; AHUS1; AMBP1; ARMD4; ARMS1; beta-1H; beta-1-H-globulin; CFHL3; complement factor H; factor H; factor H-like 1; FH; FHL1; H factor 1 (complement); H factor 2 (complement); HF; HF1; HF2; HUS
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP08603
  • Protein Name
    Complement factor H
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Factor H Polyclonal Antibody, Unconjugated (bs-9525R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Product group Antibodies
References
Bioss Antibodies
CFH Polyclonal AntibodyBS-9525R
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetCFH
  • SizePrice
Product group Antibodies
Biomatik
CFH Polyclonal AntibodyCAC13012
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetCFH
  • SizePrice
Product group Antibodies
Assay Genie
CFH AntibodyCAB13686
TargetCFH
  • SizePrice
Anti-CFH Antibody
Product group Antibodies
Antibodies.com
Anti-CFH AntibodyA30162
ApplicationsWestern Blot, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CFH Antibody144-65490
ApplicationsImmunoHistoChemistry
TargetCFH
  • SizePrice
Product group Antibodies
Biosynth
Factor H antibody20C-CR6027SP
  • SizePrice
Product group Antibodies
MedChem Express
Factor H Antibody (YA3361, Rabbit)HY-P83616
TargetCFH
  • SizePrice
Figure 1. Western blot analysis of Factor H/CFH using anti-Factor H/CFH antibody (A00562-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCP tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor H/CFH antigen affinity purified polyclonal antibody (Catalog # A00562-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Factor H/CFH at approximately 150 kDa. The expected band size for Factor H/CFH is at 139 kDa.
Product group Antibodies
Boster Bio
Anti-Factor H/CFH Antibody Picoband(r)A00562-3-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetCFH
  • SizePrice
Western blot All lanes: CFH antibody at 3microg/ml + human serum Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 140, 52 kDa Observed band size: 140 kDa
Product group Antibodies
Cusabio
CFH AntibodyCSB-PA005273ESR2HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCFH
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CFH / Complement Factor H Antibody (clone 02, Preservative Free)LS-C819837
ApplicationsELISA
TargetCFH
  • SizePrice