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Anti-ch-TOG Antibody

Anti-ch-TOG Antibody

A304698
Antibodies.com
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-ch-TOG Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to ch-TOG.
  • UNSPSC
    12352203

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Figure 7. Flow Cytometry analysis of U937 cells using anti-ch TOG/CKAP5 antibody (A05324). Overlay histogram showing U937 cells stained with A05324 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ch TOG/CKAP5 Antibody (A05324, 1 microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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