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ProductsBack/CKAP5 / ch-TOG Antibody (AP)

CKAP5 / ch-TOG Antibody (AP)

LS-C410068
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetCKAP5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CKAP5 / ch-TOG Antibody (AP)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 218kDa/225kDa/226kDa, while the observed MW by Western blot was 270kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 218kDa/225kDa/226kDa, while the observed MW by Western blot was 270kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Alk. Phosphatase
  • Estimated Purity
    ...
  • Gene ID9793
  • Target name
    CKAP5
  • Target description
    cytoskeleton associated protein 5
  • Target synonyms
    CHTOG, MSPS, TOG, TOGp, ch-TOG, cytoskeleton-associated protein 5, colonic and hepatic tumor over-expressed gene protein, colonic and hepatic tumor overexpressed gene protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 7. Flow Cytometry analysis of U937 cells using anti-ch TOG/CKAP5 antibody (A05324). Overlay histogram showing U937 cells stained with A05324 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ch TOG/CKAP5 Antibody (A05324, 1 microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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