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Immunohistochemical staining of human cerebellum shows strong positivity in cells in molecular layer.
Immunohistochemical staining of human cerebellum shows strong positivity in cells in molecular layer.
Immunohistochemical staining of human cerebellum shows strong positivity in cells in molecular layer.

Anti-CHM Antibody

HPA003231
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCHM
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CHM
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1121
  • Target name
    CHM
  • Target description
    CHM Rab escort protein
  • Target synonyms
    DXS540, GGTA, HSD-32, REP-1, TCD, rab proteins geranylgeranyltransferase component A 1, CHM, Rab escort protein 1, choroideremia (Rab escort protein 1)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP24386
  • Protein Name
    Rab proteins geranylgeranyltransferase component A 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of CHM using anti-CHM antibody (A00814-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHM antigen affinity purified polyclonal antibody (Catalog # A00814-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHM at approximately 73 kDa. The expected band size for CHM is at 100 kDa.
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