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CHM Antibody

CAB8345
Assay Genie
ReactivityHuman
Product group Antibodies
TargetCHM
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Overview

  • Supplier
    Assay Genie
  • Product Name
    CHM Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID1121
  • Target name
    CHM
  • Target description
    CHM Rab escort protein
  • Target synonyms
    DXS540, GGTA, HSD-32, REP-1, TCD, rab proteins geranylgeranyltransferase component A 1, CHM, Rab escort protein 1, choroideremia (Rab escort protein 1)
  • Host
    Rabbit
  • Protein IDP24386
  • Protein Name
    Rab proteins geranylgeranyltransferase component A 1
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CHM using anti-CHM antibody (A00814-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHM antigen affinity purified polyclonal antibody (Catalog # A00814-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHM at approximately 73 kDa. The expected band size for CHM is at 100 kDa.
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