Western blot analysis of -CIAS1/NALP3 using anti--CIAS1/NALP3 antibody (PA1665). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HEP-2 Cell Lysate Lane 2: A549 Cell Lysate Lane 3: U87 Cell Lysate Lane 4: CEM Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti--CIAS1/NALP3 antigen affinity purified polyclonal antibody (Catalog # PA1665) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for -CIAS1/NALP3 at approximately 118KD. The expected band size for -CIAS1/NALP3 is at 118KD.
Anti-CIAS1/NALP3 Antibody
PA1665
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-CIAS1/NALP3 Antibody
- Delivery Days Customer9
- Applications SupplierIHP, WB, IHC
- CertificationResearch Use Only
- ConcentrationAdd 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Estimated PurityImmunogen affinity purified.
- Reactivity SupplierHuman, Mouse, Rat, Hamster
- UNSPSC12352203