Western blot analysis of -CIAS1/NALP3 using anti--CIAS1/NALP3 antibody (PA1665). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HEP-2 Cell Lysate Lane 2: A549 Cell Lysate Lane 3: U87 Cell Lysate Lane 4: CEM Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti--CIAS1/NALP3 antigen affinity purified polyclonal antibody (Catalog # PA1665) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for -CIAS1/NALP3 at approximately 118KD. The expected band size for -CIAS1/NALP3 is at 118KD.
Western blot analysis of -CIAS1/NALP3 using anti--CIAS1/NALP3 antibody (PA1665). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HEP-2 Cell Lysate Lane 2: A549 Cell Lysate Lane 3: U87 Cell Lysate Lane 4: CEM Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti--CIAS1/NALP3 antigen affinity purified polyclonal antibody (Catalog # PA1665) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for -CIAS1/NALP3 at approximately 118KD. The expected band size for -CIAS1/NALP3 is at 118KD.
Anti-CIAS1/NALP3/NLRP3 Antibody Picoband(r)
PA1665
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetNLRP3
Overview
- SupplierBoster Bio
- Product NameAnti-CIAS1/NALP3 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
- Applications SupplierIHP, WB, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID114548
- Target nameNLRP3
- Target descriptionNLR family pyrin domain containing 3
- Target synonymsAGTAVPRL; AII; AVP; C1orf7; caterpiller protein 1.1; CIAS1; CLR1.1; cold autoinflammatory syndrome 1 protein; cold-induced autoinflammatory syndrome 1 protein; cryopyrin; cryopyrin, NACHT, LRR and PYD domains - containing protein 3; deafness, autosomal dominant 34; DFNA34; FCAS; FCAS1; FCU; KEFH; MWS; NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3; NACHT, LRR and PYD containing protein 3; NACHT, LRR and PYD domains-containing protein 3; NALP3; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3; PYPAF1; PYRIN-containing APAF1-like protein 1
- HostRabbit
- IsotypeIgG
- Protein IDQ96P20
- Protein NameNACHT, LRR and PYD domains-containing protein 3
- Scientific DescriptionBoster Bio Anti-CIAS1/NALP3/NLRP3 Antibody catalog # PA1665. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human, Mouse, Rat
- Reactivity SupplierHuman, Mouse, Rat, Hamster
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
References
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- The circadian clock protein Rev-erbalpha provides neuroprotection and attenuates neuroinflammation against Parkinsons disease via the microglial NLRP3 inflammasome. Kou L et al., 2022 Jun 6, J NeuroinflammationRead more
- Pyrroloquinoline quinone ameliorates diabetic cardiomyopathy by inhibiting the pyroptosis signaling pathway in C57BL/6 mice and AC16 cells. Qu XF et al., 2022 Jun, Eur J NutrRead more
- Isoliquiritigenin alleviates LPS/ D-GalN-induced acute liver failure by activating the PGC-1alpha/ Nrf2 pathway to reduce oxidative stress and inflammatory response. Wang L et al., 2021 Nov, Int ImmunopharmacolRead more
- LncRNA 4344 promotes NLRP3-related neuroinflammation and cognitive impairment by targeting miR-138-5p. Feng X et al., 2021 Nov, Brain Behav ImmunRead more
- Deficiency of tenascin-C attenuated cardiac injury by inactivating TLR4/NLRP3/caspase-1 pathway after myocardial infarction. Xu M et al., 2021 Oct, Cell SignalRead more
- Anti-inflammatory effect of a polysaccharide fraction from Craterellus cornucopioides in LPS-stimulated macrophages. Xu JJ et al., 2021 Jun 30, J Food BiochemRead more
- MIF inhibitor ISO-1 alleviates severe acute pancreatitis-associated acute kidney injury by suppressing the NLRP3 inflammasome signaling pathway. Liu Y et al., 2021 Jul, Int ImmunopharmacolRead more
- Polystyrene microplastics lead to pyroptosis and apoptosis of ovarian granulosa cells via NLRP3/Caspase-1 signaling pathway in rats. Hou J et al., 2021 Apr 1, Ecotoxicol Environ SafRead more
- Genistein ameliorates inflammation and insulin resistance through mediation of gut microbiota composition in type 2 diabetic mice. Yang R et al., 2021 Jun, Eur J NutrRead more