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Western blot analysis of NLRP3 using anti-NLRP3 antibody (PA1665). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thyus tissue lysates, Lane 3: mouse RAW264.7 whole cell lysates, Lane 4: mouse J774A.1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRP3 antigen affinity purified polyclonal antibody (PA1665) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NLRP3 at approximately 110 kDa. The expected band size for NLRP3 is at 118kDa.
Western blot analysis of NLRP3 using anti-NLRP3 antibody (PA1665). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thyus tissue lysates, Lane 3: mouse RAW264.7 whole cell lysates, Lane 4: mouse J774A.1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRP3 antigen affinity purified polyclonal antibody (PA1665) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NLRP3 at approximately 110 kDa. The expected band size for NLRP3 is at 118kDa.
Western blot analysis of NLRP3 using anti-NLRP3 antibody (PA1665). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thyus tissue lysates, Lane 3: mouse RAW264.7 whole cell lysates, Lane 4: mouse J774A.1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRP3 antigen affinity purified polyclonal antibody (PA1665) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NLRP3 at approximately 110 kDa. The expected band size for NLRP3 is at 118kDa.

Anti-CIAS1/NALP3/NLRP3 Antibody Picoband(r)

PA1665
Boster Bio
TargetNLRP3
DownloadDatasheet
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Product group Antibodies
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-CIAS1/NALP3 Antibody
  • Delivery Days Customer
    9
  • Antibody Specificity
    No cross reactivity with other proteins.
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications Supplier
    IHP, WB, IHC
  • Concentration
    500 ug/ml
  • Formulation
    Lyophilized
  • Gene ID114548
  • Target name
    NLRP3
  • Target description
    NLR family pyrin domain containing 3
  • Target synonyms
    AGTAVPRL; AII; AVP; C1orf7; caterpiller protein 1.1; CIAS1; CLR1.1; cold autoinflammatory syndrome 1 protein; cold-induced autoinflammatory syndrome 1 protein; cryopyrin; cryopyrin, NACHT, LRR and PYD domains - containing protein 3; deafness, autosomal dominant 34; DFNA34; FCAS; FCAS1; FCU; KEFH; MWS; NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3; NACHT, LRR and PYD containing protein 3; NACHT, LRR and PYD domains-containing protein 3; NALP3; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3; PYPAF1; PYRIN-containing APAF1-like protein 1
  • Protein IDQ96P20
  • Protein Name
    NACHT, LRR and PYD domains-containing protein 3
  • Scientific Description
    Boster Bio Anti-CIAS1/NALP3/NLRP3 Antibody catalog # PA1665. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity Supplier
    Human, Mouse, Rat, Hamster
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Epigallocatechin-3-gallate ameliorates renal endoplasmic reticulum stress-mediated inflammation in type 2 diabetic rats. Yang R et al., 2022 Aug, Exp Biol Med (Maywood)
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  • The circadian clock protein Rev-erbalpha provides neuroprotection and attenuates neuroinflammation against Parkinsons disease via the microglial NLRP3 inflammasome. Kou L et al., 2022 Jun 6, J Neuroinflammation
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  • Pyrroloquinoline quinone ameliorates diabetic cardiomyopathy by inhibiting the pyroptosis signaling pathway in C57BL/6 mice and AC16 cells. Qu XF et al., 2022 Jun, Eur J Nutr
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  • Isoliquiritigenin alleviates LPS/ D-GalN-induced acute liver failure by activating the PGC-1alpha/ Nrf2 pathway to reduce oxidative stress and inflammatory response. Wang L et al., 2021 Nov, Int Immunopharmacol
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  • LncRNA 4344 promotes NLRP3-related neuroinflammation and cognitive impairment by targeting miR-138-5p. Feng X et al., 2021 Nov, Brain Behav Immun
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  • Deficiency of tenascin-C attenuated cardiac injury by inactivating TLR4/NLRP3/caspase-1 pathway after myocardial infarction. Xu M et al., 2021 Oct, Cell Signal
    Read more
  • Anti-inflammatory effect of a polysaccharide fraction from Craterellus cornucopioides in LPS-stimulated macrophages. Xu JJ et al., 2021 Jun 30, J Food Biochem
    Read more
  • MIF inhibitor ISO-1 alleviates severe acute pancreatitis-associated acute kidney injury by suppressing the NLRP3 inflammasome signaling pathway. Liu Y et al., 2021 Jul, Int Immunopharmacol
    Read more
  • Polystyrene microplastics lead to pyroptosis and apoptosis of ovarian granulosa cells via NLRP3/Caspase-1 signaling pathway in rats. Hou J et al., 2021 Apr 1, Ecotoxicol Environ Saf
    Read more
  • Genistein ameliorates inflammation and insulin resistance through mediation of gut microbiota composition in type 2 diabetic mice. Yang R et al., 2021 Jun, Eur J Nutr
    Read more