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Anti-Clathrin Heavy Chain Antibody

Anti-Clathrin Heavy Chain Antibody

A121586
Antibodies.com
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-Clathrin Heavy Chain Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Goat
  • Isotype
    IgG
  • Scientific Description
    Goat polyclonal antibody to Clathrin Heavy Chain.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Clathrin heavy chain/CLTC using anti-Clathrin heavy chain/CLTC antibody (A03134-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Clathrin heavy chain/CLTC antigen affinity purified polyclonal antibody (Catalog # A03134-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Clathrin heavy chain/CLTC at approximately 192KD. The expected band size for Clathrin heavy chain/CLTC is at 192KD.
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