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ProductsBack/CLTC / Clathrin Heavy Chain Antibody

CLTC / Clathrin Heavy Chain Antibody

LS-C747528
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetCLTC
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CLTC / Clathrin Heavy Chain Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 187kDa/191kDa, while the observed MW by Western blot was 191kDa.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 187kDa/191kDa, while the observed MW by Western blot was 191kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1213
  • Target name
    CLTC
  • Target description
    clathrin heavy chain
  • Target synonyms
    CHC, CHC17, CLH-17, CLTCL2, Hc, MRD56, clathrin heavy chain 1, clathrin heavy chain on chromosome 17, clathrin, heavy polypeptide (Hc), clathrin, heavy polypeptide-like 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Clathrin heavy chain/CLTC using anti-Clathrin heavy chain/CLTC antibody (A03134-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Clathrin heavy chain/CLTC antigen affinity purified polyclonal antibody (Catalog # A03134-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Clathrin heavy chain/CLTC at approximately 192KD. The expected band size for Clathrin heavy chain/CLTC is at 192KD.
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Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Clathrin heavy chain Polyclonal Antibody, Unconjugated (bs-21805R) at 1:200 overnight at 4°C, DAB staining.
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