Figure 1. IHC analysis of Claudin 1/CLDN1 using anti Claudin 1/CLDN1 antibody (A01585-2). Claudin 1/CLDN1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2microg/ml rabbit anti-Claudin 1/CLDN1 Antibody (A01585-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.