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Western Blot analysis of HeLa cells using Claudin-1 Polyclonal Antibody
Western Blot analysis of HeLa cells using Claudin-1 Polyclonal Antibody
Western Blot analysis of HeLa cells using Claudin-1 Polyclonal Antibody

CLDN1 Antibody

CSB-PA001656
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCLDN1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CLDN1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9076
  • Target name
    CLDN1
  • Target description
    claudin 1
  • Target synonyms
    claudin-1; CLD1; ILVASC; SEMP1; senescence-associated epithelial membrane protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95832
  • Protein Name
    Claudin-1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. IHC analysis of Claudin 1/CLDN1 using anti Claudin 1/CLDN1 antibody (A01585-2). Claudin 1/CLDN1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2microg/ml rabbit anti-Claudin 1/CLDN1 Antibody (A01585-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Western blot analysis in human cell lines RT-4 and U-251MG using Anti-CLDN1 antibody. Corresponding CLDN1 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
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