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Anti-CLEC7A (N-term) Antibody

102-21449
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCLEC7A
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-CLEC7A (N-term) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB30231
  • Gene ID64581
  • Target name
    CLEC7A
  • Target description
    C-type lectin domain containing 7A
  • Target synonyms
    beta-glucan receptor; BGR; CANDF4; CD369; CLECSF12; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 12; C-type lectin domain family 7 member A; C-type lectin superfamily member 12; DC-associated C-type lectin 1; DECTIN1; dectin-1; dendritic cell-associated C-type lectin-1; lectin-like receptor 1; SCARE2
  • Host
    Rabbit
  • Protein IDQ9BXN2
  • Protein Name
    C-type lectin domain family 7 member A
  • Scientific Description
    Rabbit Anti-CLEC7A (N-term) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Dectin-1/CLEC7A using anti-Dectin-1/CLEC7A antibody (A02731-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dectin-1/CLEC7A antigen affinity purified polyclonal antibody (Catalog # A02731-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dectin-1/CLEC7A at approximately 36 kDa. The expected band size for Dectin-1/CLEC7A is at 28 kDa.
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