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CLEC7A / Dectin 1 Antibody

LS-C498051
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCLEC7A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CLEC7A / Dectin 1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 5kDa/8kDa/13-27kDa, while the observed MW by Western blot was 31kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 5kDa/8kDa/13-27kDa, while the observed MW by Western blot was 31kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2.71 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID64581
  • Target name
    CLEC7A
  • Target description
    C-type lectin domain containing 7A
  • Target synonyms
    BGR, CANDF4, CD369, CLECSF12, DECTIN1, SCARE2, C-type lectin domain family 7 member A, C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 12, C-type lectin superfamily member 12, DC-associated C-type lectin 1, beta-glucan receptor, dectin-1, dendritic cell-associated C-type lectin-1, lectin-like receptor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Dectin-1/CLEC7A using anti-Dectin-1/CLEC7A antibody (A02731-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dectin-1/CLEC7A antigen affinity purified polyclonal antibody (Catalog # A02731-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dectin-1/CLEC7A at approximately 36 kDa. The expected band size for Dectin-1/CLEC7A is at 28 kDa.
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