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Immunohistochemical staining of human pituitary gland shows strong cytoplasmic positivity in anterior cells.
Immunohistochemical staining of human pituitary gland shows strong cytoplasmic positivity in anterior cells.
Immunohistochemical staining of human pituitary gland shows strong cytoplasmic positivity in anterior cells.

Anti-CNR1 Antibody

HPA069945
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetCNR1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CNR1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1268
  • Target name
    CNR1
  • Target description
    cannabinoid receptor 1
  • Target synonyms
    CANN6; cannabinoid receptor 1; cannabinoid receptor 1 (brain); CB1; CB1A; CB1K5; CB1R; CB-R; central cannabinoid receptor; CNR
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21554
  • Protein Name
    Cannabinoid receptor 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western Blot Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, 293 whole cell lysate All lanes: CNR1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 46, 50 kDa Observed band size: 53 kDa
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Figure 1. Western blot analysis of Cannabinoid Receptor I using anti-Cannabinoid Receptor I antibody (A01291-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cannabinoid Receptor I antigen affinity purified polyclonal antibody (Catalog # A01291-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cannabinoid Receptor I at approximately 60KD. The expected band size for Cannabinoid Receptor I is at 53KD.
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