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CNR1 / CB1 Antibody

LS-C667732
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetCNR1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CNR1 / CB1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western blot analysis of CB1 expression in mouse brain (A) whole cell lysates.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) Western blot analysis of CB1 expression in mouse brain (A) whole cell lysates.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1268
  • Target name
    CNR1
  • Target description
    cannabinoid receptor 1
  • Target synonyms
    CANN6; cannabinoid receptor 1; cannabinoid receptor 1 (brain); CB1; CB1A; CB1K5; CB1R; CB-R; central cannabinoid receptor; CNR
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, 293 whole cell lysate All lanes: CNR1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 46, 50 kDa Observed band size: 53 kDa
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Figure 1. Western blot analysis of Cannabinoid Receptor I using anti-Cannabinoid Receptor I antibody (A01291-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cannabinoid Receptor I antigen affinity purified polyclonal antibody (Catalog # A01291-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cannabinoid Receptor I at approximately 60KD. The expected band size for Cannabinoid Receptor I is at 53KD.
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