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Western Blot Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, 293 whole cell lysate All lanes: CNR1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 46, 50 kDa Observed band size: 53 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, 293 whole cell lysate All lanes: CNR1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 46, 50 kDa Observed band size: 53 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, 293 whole cell lysate All lanes: CNR1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 46, 50 kDa Observed band size: 53 kDa

CNR1 Antibody

CSB-PA005678LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetCNR1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CNR1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1268
  • Target name
    CNR1
  • Target description
    cannabinoid receptor 1
  • Target synonyms
    CANN6, CB-R, CB1, CB1A, CB1K5, CB1R, CNR, cannabinoid receptor 1, cannabinoid receptor 1 (brain), central cannabinoid receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21554
  • Protein Name
    Cannabinoid receptor 1
  • Scientific Description
    Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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SH-SY5Y cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CNR1 Polyclonal Antibody(bs-1683R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Figure 1. Western blot analysis of Cannabinoid Receptor I using anti-Cannabinoid Receptor I antibody (A01291-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cannabinoid Receptor I antigen affinity purified polyclonal antibody (Catalog # A01291-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cannabinoid Receptor I at approximately 60KD. The expected band size for Cannabinoid Receptor I is at 53KD.
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Immunohistochemical staining of human pituitary gland shows strong cytoplasmic positivity in anterior cells.
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