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Immunofluorescence staining of mouse brain cortex shows strong cytoplasmic positivity in neuropil.
Immunofluorescence staining of mouse brain cortex shows strong cytoplasmic positivity in neuropil.
Immunofluorescence staining of mouse brain cortex shows strong cytoplasmic positivity in neuropil.

Anti-CNTN1 Antibody

HPA070467
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCNTN1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CNTN1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1272
  • Target name
    CNTN1
  • Target description
    contactin 1
  • Target synonyms
    contactin-1; F3; glycoprotein gP135; GP135; MYPCN; neural cell surface protein F3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12860
  • Protein Name
    Contactin-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Contactin 1/CNTN1 using anti-Contactin 1/CNTN1 antibody (A04538-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Contactin 1/CNTN1 antigen affinity purified polyclonal antibody (Catalog # A04538-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Contactin 1/CNTN1 at approximately 135 kDa. The expected band size for Contactin 1/CNTN1 is at 113 kDa.
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