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Western Blot analysis of mouse brain cells using Contactin 1 Polyclonal Antibody
Western Blot analysis of mouse brain cells using Contactin 1 Polyclonal Antibody
Western Blot analysis of mouse brain cells using Contactin 1 Polyclonal Antibody

CNTN1 Antibody

CSB-PA004825
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCNTN1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CNTN1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1272
  • Target name
    CNTN1
  • Target description
    contactin 1
  • Target synonyms
    contactin-1; F3; glycoprotein gP135; GP135; MYPCN; neural cell surface protein F3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12860
  • Protein Name
    Contactin-1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Contactin 1/CNTN1 using anti-Contactin 1/CNTN1 antibody (A04538-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Contactin 1/CNTN1 antigen affinity purified polyclonal antibody (Catalog # A04538-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Contactin 1/CNTN1 at approximately 135 kDa. The expected band size for Contactin 1/CNTN1 is at 113 kDa.
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Immunofluorescence staining of mouse brain cortex shows strong cytoplasmic positivity in neuropil.
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