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ProductsBack/Goat anti-contactin 1

Goat anti-contactin 1

EB12273
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCNTN1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-contactin 1 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID1272
  • Target name
    CNTN1
  • Target description
    contactin 1
  • Target synonyms
    contactin-1; F3; glycoprotein gP135; GP135; MYPCN; neural cell surface protein F3
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001834.2; NP_778203.1; NP_001242992.1. GeneIDs all Nonhuman: 12805 (mouse); 117258 (rat);. Purification: Antigen affinity purified. Names and symbols: CNTN1; contactin 1; F3; GP135; contactin-1; glycoprotein gP135; neural cell surface protein F3
  • Reactivity Supplier
    Human, Mouse, Rat, Pig
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Contactin 1/CNTN1 using anti-Contactin 1/CNTN1 antibody (A04538-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Contactin 1/CNTN1 antigen affinity purified polyclonal antibody (Catalog # A04538-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Contactin 1/CNTN1 at approximately 135 kDa. The expected band size for Contactin 1/CNTN1 is at 113 kDa.
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