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Immunohistochemical staining of human retina shows cytoplasmic positivity in cells in inner nuclear layer and nerve fibers.
Immunohistochemical staining of human retina shows cytoplasmic positivity in cells in inner nuclear layer and nerve fibers.
Immunohistochemical staining of human retina shows cytoplasmic positivity in cells in inner nuclear layer and nerve fibers.

Anti-CRABP1 Antibody

HPA017203
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetCRABP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CRABP1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1381
  • Target name
    CRABP1
  • Target description
    cellular retinoic acid binding protein 1
  • Target synonyms
    cellular retinoic acid-binding protein 1; cellular retinoic acid-binding protein I; CRABP; CRABPI; CRABP-I; RBP5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29762
  • Protein Name
    Cellular retinoic acid-binding protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CRABP1 using anti-CRABP1 antibody (A07951-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRABP1 antigen affinity purified polyclonal antibody (Catalog # A07951-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRABP1 at approximately 16 kDa. The expected band size for CRABP1 is at 16 kDa.
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Western blot All lanes: CRABP1 antibody at 4microg/ml Lane 1: Mouse eye tissue Lane 2: THP-1 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa
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