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Western blot All lanes: CRABP1 antibody at 4microg/ml Lane 1: Mouse eye tissue Lane 2: THP-1 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa
Western blot All lanes: CRABP1 antibody at 4microg/ml Lane 1: Mouse eye tissue Lane 2: THP-1 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa
Western blot All lanes: CRABP1 antibody at 4microg/ml Lane 1: Mouse eye tissue Lane 2: THP-1 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa

CRABP1 Antibody

CSB-PA005935DA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCRABP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CRABP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1381
  • Target name
    CRABP1
  • Target description
    cellular retinoic acid binding protein 1
  • Target synonyms
    CRABP, CRABP-I, CRABPI, RBP5, cellular retinoic acid-binding protein 1, cellular retinoic acid-binding protein I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29762
  • Protein Name
    Cellular retinoic acid-binding protein 1
  • Scientific Description
    Cytosolic CRABPs may regulate the access of retinoic acid to the nuclear retinoic acid receptors.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CRABP1 using anti-CRABP1 antibody (A07951-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRABP1 antigen affinity purified polyclonal antibody (Catalog # A07951-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRABP1 at approximately 16 kDa. The expected band size for CRABP1 is at 16 kDa.
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