Western blot analysis of CRISPR-Cas9 SP expression in (1) 293T cell lysate transfected with CRISPR-Cas9; (2) 293T cell lysate; (3) 3T3 cell lysate; (4) PC12 cell lysate.
Western blot analysis of CRISPR-Cas9 SP expression in (1) 293T cell lysate transfected with CRISPR-Cas9; (2) 293T cell lysate; (3) 3T3 cell lysate; (4) PC12 cell lysate.
Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody
M30929
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
Targetcas9
Overview
- SupplierBoster Bio
- Product NameAnti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDABCI-3
- Gene ID901176
- Target namecas9
- Target descriptiontype II CRISPR RNA-guided endonuclease Cas9
- Target synonymsSPY_RS04360, SPy1046, SPy_1046, type II CRISPR RNA-guided endonuclease Cas9
- HostRabbit
- IsotypeIgG
- Protein IDQ99ZW2
- Protein NameCRISPR-associated endonuclease Cas9/Csn1
- Scientific DescriptionBoster Bio Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody catalog # M30929. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Recombinant protein.
- Storage Instruction-20°C
- UNSPSC12352203
References
- Wang Z, Yuan H, Yang L, et al. Decreasing predictable DNA off-target effects and narrowing editing windows of adenine base editors by fusing human Rad18 protein variant. Int J Biol Macromol. 2023,253(Pt 7):127418. doi: 10.1016/j.ijbiomac.2023.127418Read this paper
Datasheet
MSDS
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![IP analysis was performed with lysate from HEK293T cells expressing Flag-tagged S. pyogenes Cas9 and either Cas9 antibody [7A9] followed by incubation with a 1:1 mixture of protein A/G sepharose beads (Cas9 Sup) or Cas9 antibody [7A9] crosslinked with a 1:1 mixture of protein A/G sepharose beads (Cas9 X-link). The precipitates were detected by the same antibody. Asterisk indicates IgG heavy chain.](https://www.genetex.com/upload/website/prouct_img/normal/GTX53807/GTX53807_IP_w_23060900_531.webp)
Product group Antibodies
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