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Lane 1: 293 cells lysates transfected with CRISPR-Cas9; Lane 2: 293T cells lysates transfected with CRISPR-Cas9; Probed with CRISPR-Cas9 SP (6C1) Monoclonal Antibody (bsm-52960R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C._x000D_
Lane 1: 293 cells lysates transfected with CRISPR-Cas9; Lane 2: 293T cells lysates transfected with CRISPR-Cas9; Probed with CRISPR-Cas9 SP (6C1) Monoclonal Antibody (bsm-52960R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C._x000D_
Lane 1: 293 cells lysates transfected with CRISPR-Cas9; Lane 2: 293T cells lysates transfected with CRISPR-Cas9; Probed with CRISPR-Cas9 SP (6C1) Monoclonal Antibody (bsm-52960R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C._x000D_

CRISPR-Cas9 Recombinant Antibody

BSM-52960R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBacteria, Other Species
Targetcas9
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Overview

  • Supplier
    Bioss
  • Product Name
    CRISPR-Cas9 Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID901176
  • Target name
    cas9
  • Target description
    type II CRISPR RNA-guided endonuclease Cas9
  • Target synonyms
    SPY_RS04360, SPy1046, SPy_1046, type II CRISPR RNA-guided endonuclease Cas9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99ZW2
  • Protein Name
    CRISPR-associated endonuclease Cas9/Csn1
  • Reactivity
    Bacteria, Other Species
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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IP analysis was performed with lysate from HEK293T cells expressing Flag-tagged S. pyogenes Cas9 and either Cas9 antibody [7A9] followed by incubation with a 1:1 mixture of protein A/G sepharose beads (Cas9 Sup) or Cas9 antibody [7A9] crosslinked with a 1:1 mixture of protein A/G sepharose beads (Cas9 X-link). The precipitates were detected by the same antibody. Asterisk indicates IgG heavy chain.
Product group Antibodies
References
GeneTex
Cas9 antibody [7A9]GTX53807
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Product group Antibodies
Cusabio
cas9 AntibodyCSB-PA210245XA01FNQ
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Western blot analysis of CRISPR-Cas9 SP expression in (1) 293T cell lysate transfected with CRISPR-Cas9; (2) 293T cell lysate; (3) 3T3 cell lysate; (4) PC12 cell lysate.
Product group Antibodies
References
Boster Bio
Anti-CRISPR-Cas9 SP Rabbit Monoclonal AntibodyM30929
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Targetcas9
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