IP analysis was performed with lysate from HEK293T cells expressing Flag-tagged S. pyogenes Cas9 and either Cas9 antibody [7A9] followed by incubation with a 1:1 mixture of protein A/G sepharose beads (Cas9 Sup) or Cas9 antibody [7A9] crosslinked with a 1:1 mixture of protein A/G sepharose beads (Cas9 X-link). The precipitates were detected by the same antibody. Asterisk indicates IgG heavy chain.
![ICC/IF analysis of HeLa cells transiently transfected with flag-tagged Cas9 using Cas9 antibody [7A9] at a dilution of 0.5 μg/ml (green) and DAPI (blue).](https://www.genetex.com/upload/website/prouct_img/normal/GTX53807/GTX53807_ICCIF_w_23060900_242.webp "ICC/IF analysis of HeLa cells transiently transfected with flag-tagged Cas9 using Cas9 antibody [7A9] at a dilution of 0.5 μg/ml (green) and DAPI (blue).")
IP analysis was performed with lysate from HEK293T cells expressing Flag-tagged S. pyogenes Cas9 and either Cas9 antibody [7A9] followed by incubation with a 1:1 mixture of protein A/G sepharose beads (Cas9 Sup) or Cas9 antibody [7A9] crosslinked with a 1:1 mixture of protein A/G sepharose beads (Cas9 X-link). The precipitates were detected by the same antibody. Asterisk indicates IgG heavy chain.
Cas9 antibody [7A9]
GTX53807
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityBacteria
Targetcas9
Overview
- SupplierGeneTex
- Product NameCas9 antibody [7A9]
- Delivery Days Customer9
- Application Supplier NoteWestern Blotting Analysis: 0.5 microg/mL from a representative lot detected Cas9 in 1 microg of Myc-Cas9 expressing HEK293 cell lysate. Immunoprecipitation Analysis: A representative lot immunoprecipitated Cas9 in Flag-Cas9 and Myc-Cas9 expressing cell lysate (performed by an independent laboratory).
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID7A9
- ConjugateUnconjugated
- Gene ID901176
- Target namecas9
- Target descriptiontype II CRISPR RNA-guided endonuclease Cas9
- Target synonymsSPY_RS04360, SPy1046, SPy_1046, type II CRISPR RNA-guided endonuclease Cas9
- HostMouse
- IsotypeIgG1
- ReactivityBacteria
- Storage Instruction2°C to 8°C
- UNSPSC41116161
References
- CRISPR screening of porcine sgRNA library identifies host factors associated with Japanese encephalitis virus replication. Zhao C et al., 2020 Oct 14, Nat CommunRead this paper
- Synthetic switch to minimize CRISPR off-target effects by self-restricting Cas9 transcription and translation. Shen CC et al., 2019 Feb 20, Nucleic Acids ResRead this paper
- Inactivation of DNA repair triggers neoantigen generation and impairs tumour growth. Germano G et al., 2017 Dec 7, NatureRead this paper
- An inducible CRISPR-ON system for controllable gene activation in human pluripotent stem cells. Guo J et al., 2017 May, Protein CellRead this paper
Datasheet
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