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Immunohistochemical staining of human retina shows strong nuclear positivity in photoreceptor cells.
Immunohistochemical staining of human retina shows strong nuclear positivity in photoreceptor cells.
Immunohistochemical staining of human retina shows strong nuclear positivity in photoreceptor cells.

Anti-CRX Antibody

HPA036763
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCRX
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CRX Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1406
  • Target name
    CRX
  • Target description
    cone-rod homeobox
  • Target synonyms
    CORD2, CRD, LCA7, OTX3, cone-rod homeobox protein, orthodenticle homeobox 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43186
  • Protein Name
    Cone-rod homeobox protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of CORD2 using anti-CORD2 antibody (A02202-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CORD2 antigen affinity purified polyclonal antibody (Catalog # A02202-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CORD2 at approximately 37KD. The expected band size for CORD2 is at 32KD.
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