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Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CORD2 antibody (GTX124188) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CORD2 antibody (GTX124188) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CORD2 antibody (GTX124188) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CORD2 antibody

GTX124188
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetCRX
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Overview

  • Supplier
    GeneTex
  • Product Name
    CORD2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1406
  • Target name
    CRX
  • Target description
    cone-rod homeobox
  • Target synonyms
    CORD2, CRD, LCA7, OTX3, cone-rod homeobox protein, orthodenticle homeobox 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43186
  • Protein Name
    Cone-rod homeobox protein
  • Scientific Description
    The protein encoded by this gene is a photoreceptor-specific transcription factor which plays a role in the differentiation of photoreceptor cells. This homeodomain protein is necessary for the maintenance of normal cone and rod function. Mutations in this gene are associated with photoreceptor degeneration, Leber congenital amaurosis type III and the autosomal dominant cone-rod dystrophy 2. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Afanasyeva TAV, Corral-Serrano JC, Garanto A, et al. A look into retinal organoids: methods, analytical techniques, and applications. Cell Mol Life Sci. 2021,78(19-20):6505-6532. doi: 10.1007/s00018-021-03917-4
    Read this paper
  • Zhu J, Lamba DA. Small Molecule-Based Retinal Differentiation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells. Bio Protoc. 2018,8(12):pii: e2882. doi: 10.21769/BioProtoc.2882.
    Read this paper

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Figure 1. Western blot analysis of CORD2 using anti-CORD2 antibody (A02202-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CORD2 antigen affinity purified polyclonal antibody (Catalog # A02202-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CORD2 at approximately 37KD. The expected band size for CORD2 is at 32KD.
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