Anti-CTLA4 [1B8]

Absolute Antibody

Anti-CTLA4 [1B8]

9

This antibody is specific for CTLA-4. CTLA-4 is an inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their co

ELISA was preformed on murine CTLA-4lg to test the binding specificity of the Antibody (Walunas et al, 1994; pmid:7882171). For flow cytometry on A20, M12.41 (B cells), L cell (fibroblast), and P815 (mastocytoma) cells this antibody was used (Perkins et al, 1996; pmid:8666782). This antibody was used for blocking in mice. It prevented T-cell proliferation (Leach et al, 1996; pmid:8596936). Western blot was preformed on LN cells harvested from SJL mice using this antibody (Aruna et al, 2005; pmid:16014414). This antibody was used for flow cytometry on LN cells harvested from SJL mice (Aruna et al, 2005; pmid:16014414). Mouse CD4+ CD25+ T cells were used for a flow cytomic experiment using this antibody (Valsazina et al, 2006; pmid:16618776). Thymi from Tg4 CTLA-4WT and B10.PL mice were immunostained with this antibody (Verhagen et al, 2012; pmid:23267099). LN cells from mice were immunostained with this antibody (Paas-Rozner et al, 2003; pmid:12743364). Cultured CD4+CD25+ Tregs of mice were used in an flow cytometry experiment in which this antibody was used (Wang et al, 2010; pmid:20843956).

Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

1B8

Ctla4

cytotoxic T-lymphocyte-associated protein 4

Cd152; CD152 antigen; Ctla; Ctla-4; cytotoxic T-lymphocyte protein 4; cytotoxic T-lymphocyte-associated antigen 4; Ly-5; Ly-56

Mouse

IgG1

Cytotoxic T-lymphocyte protein 4

This chimeric mouse antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

Mouse

-20°C,2°C to 8°C

12352203