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Immunohistochemical staining of human small intestine shows strong membranous immunoreactivity in the epithelial cells.
Immunohistochemical staining of human small intestine shows strong membranous immunoreactivity in the epithelial cells.
Immunohistochemical staining of human small intestine shows strong membranous immunoreactivity in the epithelial cells.

Anti-CTNNB1 Antibody

AMAB91210
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCTNNB1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CTNNB1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL3691
  • Conjugate
    Unconjugated
  • Gene ID1499
  • Target name
    CTNNB1
  • Target description
    catenin beta 1
  • Target synonyms
    CTNNB, EVR7, MRD19, NEDSDV, armadillo, catenin beta-1, catenin (cadherin-associated protein), beta 1, 88kDa
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP35222
  • Protein Name
    Catenin beta-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human T47D whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNB1 antigen affinity purified polyclonal antibody (Catalog # A00004) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTNNB1 at approximately 95 kDa. The expected band size for CTNNB1 is at 95 kDa.
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